منابع مشابه
Dog Tear Film Proteome In-Depth Analysis.
In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of...
متن کاملQuantitative analysis of tear film fluorescence and discomfort during tear film instability and thinning.
PURPOSE The purpose of this study was to test the association between tear film fluorescence changes during tear break-up (TBU) or thinning and the concurrent ocular sensory response. METHODS Sixteen subjects kept one eye open as long as possible (MBI), indicated their discomfort level continuously, and rated ocular sensations of irritation, stinging, burning, pricking, and cooling using visu...
متن کاملUpdate on Human Tear Proteome
Tear film is a thin layer of non-newtonian fluid covering and protecting cornea and conjunctiva epithelia. The multifaced functions of tears are reflected by their complex structure and advances in proteomics/metabolomics/lipidomics technologies have greatly deepened the knowledge of the chemical composition of tears. The purpose of this article is to survey recent advances in proteomic analysi...
متن کاملProteomic analysis of the tear film in patients with keratoconus
PURPOSE To identify proteins differentially expressed between the tear film of keratoconus (KC) patients and control subjects using two dimensional electrophoresis (2-DE) and mass spectrometry-based techniques. METHODS Twenty two patients (44 eyes) diagnosed with bilateral KC and 22 control subjects (44 eyes) were studied in a prospective case-control study. Keratoconus screening programs and...
متن کاملTear secretion and tear film function in insulin dependent diabetics.
BACKGROUND Diabetic patients often complain of dry eye symptoms, such as burning and/or foreign body sensation. The aim of the present study was to investigate whether diabetes mellitus is correlated with tear film dysfunction and/or tear hyposecretion. METHODS In 86 consecutive insulin dependent diabetics with retinopathy and 84 non-diabetic controls (age and sex matched) we performed fluoro...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2015
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0144242